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Among 41 bone marrow biopsy samples from CML patients, 32% exhibited AQP5 expression (Figure 1D).
Then, the protein expression level of AQP5 was examined with bone marrow biopsy samples by immunohistochemisty (Table 1, Table S1).
While normal bone marrow biopsy samples (n = 5) showed no expression of AQP5, 32% of CML patient samples (n = 41) demonstrated AQP5 expression.
While all the normal bone marrow biopsy samples (n = 5) showed no expression of AQP5 (Figure 1C), 13 out of 41 (32%) CML patient samples demonstrated AQP5 expression (Figure 1D).
Morulae and A. phagocytophilum DNA were also detected in bone marrow biopsy samples (6, 7 ).
We obtained bone marrow biopsy samples from selected persons who exhibited the symptoms listed above.
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Cytogenetic response was assessed by G-banding and counting Ph-positive metaphases in at least 20 cells in metaphase per bone marrow biopsy sample.
No bone marrow biopsy sample was available from patient with SLIT2 and NRP1 mutations.
The possibility of hemophagocytosis was considered, and a bone marrow biopsy sample was obtained on day 4.
A bone marrow biopsy sample indicated isolated erythroblastopenia with no abnormality of other cell lineages (PCR for parvovirus B19 was negative).
Detection of the measles genome and isolated erythroblastopenia in the bone marrow biopsy sample is consistent with reports that measles virus can infect erythroid progenitors and interfere indirectly with hematopoiesis (7, 8 ).
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