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The estimated collective dose to red bone marrow, based on a one year lag period, was 4000 Gy, and the average estimated red bone marrow dose was 4.6 mGy per scan.
Therefore, the established muscle-based model (4) can derive the contrast concentration in bone marrow based on that in muscle, which also reflects the contrast dynamics in the artery.
Here we report the development of an in vitro artificial bone marrow based on a 3D scaffold with inverted colloidal crystal (ICC) geometry mimicking the structural topology of actual bone marrow matrix.
We previously established a method for isolating highly enriched MSCs and osteo-progenitors from adult murine bone marrow based on their expression of PDGFRα and Sca-1.
Hematopoietic stem cells (HSC) have high ALDH1 activity and can be isolated from bone marrow based on that unique trait [27], [28].
To this end, we first biotin-labeled cells in vivo and prospectively FACS purified HSCs from the bone marrow based on their unique cell surface phenotype (experimental strategy outlined in Fig. 1D).
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Compared to most pathways, maturation of the immune system is delayed and occurs between four and eight weeks in spleen and bone-marrow based on human B- and T-cell signatures (Fig. 3b).
Whole bone marrow cells or selected populations of marrow cells, based on their surface markers, were injected intravenously by tail vein into each recipient.
A fixed ratio between blood and bone marrow has been used in bone marrow dosimetry based on blood radioactivity levels [ 10, 64].
They isolated bone marrow cells based on size and found that while none of the larger cells were multipotent, not all of the smaller cells were multipotent, so size alone cannot be used to distinguish MSCs.
The aim of our study was to develop a novel method for bone marrow dosimetry based on planar post-therapy imaging, without the need for blood and urine sampling, and to investigate a possible correlation with haematological toxicity.
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