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The assay was able to detect parasite DNA from the liver and spleen of L. donovani-infected hamsters as well as lesion aspirates from parasitologically confirmed post-kala-azar dermal leishmaniasis (PKDL) and bone marrow aspirates from visceral leishmaniasis (VL) patients.
(1) To safely obtain bone marrow aspirates from the distal femur during arthroscopic knee surgery, (2) to purify and efficiently concentrate connective tissue progenitor cells (CTPs) in the operating room (OR), and (3) to confirm that these are CTPs through their ability to differentiate into bone cells.
Myeloma cells were obtained from heparinized bone marrow aspirates from patients with active MM during scheduled clinic visits.
Human bone marrow aspirates from healthy human adult volunteers (20 35 years of age) were purchased from AllCells LLC and cultured as reported previously [21].
hMSCs were obtained from bone marrow aspirates from Cambrex Bio Science Walkersville, Inc., from a 25 yr old healthy male and prepared as we have previously reported [57].
Primary human MSC derived from bone marrow (MSC-BM) were established from bone marrow aspirates from four donors as described previously [16].
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A bone marrow aspirate from the femur was also performed.
Components of the bone marrow aspirate from the two iliac crests were processed under sterile conditions.
A bone marrow aspirate from another patient in our study did not reveal bacterial 16S rDNA sequences.
Due to the nature of our biological samples (skin biopsy and bone marrow aspirate from patient II-2) repetitive sampling was not possible or useful.
Bone marrow aspirate from each consented donor was collected and sent to the GMP-compliant facility of Catholic Institute of Cell Therapy (Seoul, Korea, http://www.cic.re.kr) for the isolation, expansion, and quality control of human marrow-derived mesenchymal stem cells (hBM-MSCs).
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