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We have monitored patients with primary breast cancer using sequential bone marrow aspirates for several years.
Bone marrow aspirates for DTC assessment were obtained directly before primary breast cancer surgery in all patients.
Given that apoptosis is the principle mechanism of chemotherapy-induced regression [ 29], we analyzed the bone marrow aspirates for the presence of apoptotic DTC by immunocytochemical staining with M30 antibody.
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A sternal bone marrow aspirate for Leishmania DNA detection by nested polymerase chain reaction (n-PCR) was performed.
He has been monitored closely, including regular checks of bone marrow aspirate for early detection of clonal myeloid proliferation.
Bone marrow aspirate for gene expression studies was taken on the day of inclusion and 1 3 days after end of intake of the study product.
The gold standard for making a diagnosis of iron deficiency anaemia examination of stained bone marrow aspirate for haemosiderin is invasive.
At least four slides of bone marrow aspirate for each patient were evaluated independently and blindly by two observers (F Pasini and G Pelosi) without knowledge of patient's identity or clinical course of the disease.
Baseline evaluations included physical examination, blood counts, hepatic and renal function tests, bone marrow aspirate for centrally performed (PL) molecular cytogenetic analyses (fluorescence in situ hybridization) and biopsy, serum and urine protein electrophoreses, and quantitation of serum immunoglobulins and urinary light chains, β2M and C reactive protein.
In addition, both indirect fluorescence antibody test (IFAT) and sternal bone marrow aspirate for Leishmania DNA detection by n-PCR were performed at T0 and T12 in IMMD and SD groups and at T0 in healthy dog control (see also " Serological and molecular assays" paragraph).
MSC from human bone marrow were either isolated from bone marrow aspirates from healthy donors for allogeneic transplantation (BM) or from the caput femoris from elderly patients undergoing femoral head prosthesis (HIP) [65].
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