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Mouse marrow MSCs were collected, cultured, and passaged for three passages and then identified by morphology.
Rat bone marrow MSCs were transfected by lentiviral vectors with the GFP-Bcl-2 gene (GFP-Bcl-2-MSCs).
Human bone marrow MSCs were cultured on the hydrogels with different stiffness under adipogenic and osteogenic conditions.
The primary cultured mouse marrow MSCs were labeled with amino-modified FMNPs then intravenously injected into mouse model with subcutaneous gastric tumor, and then, the in vivo distribution of FMNP-labeled MSCs was observed by using fluorescence imaging system and magnetic resonance imaging system.
To obtain primary cultures, human bone marrow MSCs were derived from iliac crest marrow aspirates of healthy donor.
Primary MSCs derived from human fetal bone marrow were transfected with a retroviral vector encoding v-myc oncogene (Figure 1A), and clones of immortalized human bone marrow MSCs were generated (see Methods).
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These results indicate that synovial MSCs and bone marrow MSCs are useful cell sources for cartilage regeneration, but it is easier to prepare a sufficient number in synovial MSCs than in bone marrow MSCs when autologous serum is used.
A written consent for use of the bone marrow MSCs was presented by the donor.
Bone marrow MSCs are skeletal stem cells that give rise to osteoblastic lineage of cells.
In particular, bone marrow MSCs are comprehensively characterized and represent promising candidates [ 5].
Thus, LRP6 in bone marrow MSCs is essential for their survival and proliferation, and therefore, is a key positive regulator for bone formation during skeletal growth and remodeling.
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