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We repeated the experiment using bone marrow MSCs from three rabbits.
Bone marrow MSCs from the mutant mice showed decreased colony forming, cell viability and cell proliferation.
More than 10 million synovial MSCs were obtained from all nine donors, contrary to more than 1 million bone marrow MSCs from only two among nine donors [ 19].
Unlike bone marrow, MSCs from other tissues can be easily obtained by non-invasive methods and its proliferation can be maintained up to many passages [ 34, 35].
When human bone marrow MSCs from OA patients were cultured in a 3-D polyglycolic acid scaffold in the presence of TGF-β3, upregulated expression of collagen type X was significantly suppressed by the presence of PTHrP whereas expression of other cartilage-specific matrix proteins was not affected [ 36].
In the present study, the significantly altered functions of bone marrow MSCs from the mutant mice in the in vitro culture experiments strongly suggest that the functional decline of bone marrow MSCs after LRP6 deletion contributes directly to the skeletal defects in the mutant mice.
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Conversely, in bone marrow MSC from Hmgb2-/ mice, Col10a1 was more strongly expressed than in wildtype MSC.
Compared with those from bone marrow, MSCs derived from UC have higher proliferative potency, stronger differentiation capacity, and lower risk for viral contamination.
Similarly, Kafienah and colleagues [ 35] have studied whether it is possible to engineer hyaline cartilage using bone marrow MSCs derived from patients with hip OA.
Bone marrow MSCs isolated from human ribs were differentiated down the adipocyte lineage (mBM-Ad) by culture in mesenchymal stem cell basal media containing hydrocortisone and specific PUFAs.
Moreover, the group subsequently demonstrated that catheter-based endomyocardial injections of autologous and allogeneic bone marrow MSCs, ranging from 20 million to 200 million in cell number, were similarly effective as the intervention against ischemic heart failure [ 24]; these manifestations raised the enthusiasm for allogeneic transplantation.
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