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This event leads to enhanced ubiquitination and p27 proteolysis by the proteasome, which marks the restriction point and promotes cell proliferation [ 5].
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The primers P1 (5'-GGTTCAGGAGGTGGCTCTGGTGGATCGCTCACCCCGGAACAAGTT-3') and P2 (5'-CGCTCGAGAGCCGTCGTCTTCTTTGCCGT-3') (the double line marks the XhoI restriction site) were used to amplify the PhaP gene.
The primers B1 (5'-GC GGAtheATGGCTCCTCTGAAGATTC-3') (the double line marks the BamHI restriction site) and B2 (5'-ACCAGAGCCACCTCCTGAACCGCCTCCACCTGTAATCCAAGGAATG-3') were used to amplify extracellular domain of B7-2 coding sequence.
This means that we can restrict our attention to restriction-free processes built over Act(_{gamma }), as a restricted process ({(mathbf{nu } L }t) in (mathcal {P}) is mapped via ({mathrm{dec}}) to the same marking of the restriction-free process (t{{L'/L}}) in (mathcal {P}^gamma ).
Indeed, it appears that the major function of lentiviral accessory proteins is to act as adaptor molecules that recruit ubiquitination machinery to mark the targeted restriction factor for proteasome-mediated degradation (Schwefel et al., 2014).
If RB k is marked for restriction in the restriction list of sector s, then z s,k is equal to the utility (u^{2}_{x,k}).
To mark this restriction, we will let represented kinds be denoted by K's, rather than our usual X's.
Many European countries have demonstrated marked decreases in antimicrobial resistance after the restriction of antimicrobial use in food animals.
Then, the RFLP availability of the restriction enzymes for the input SNP-containing sequence (marked as +) and its complementary sequence (marked as -) is shown separately in Figure 3B.
This interchange marks the eastern end of the cargo restrictions.
That's the restriction".
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