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Dotted line marks the mid-Cen position.
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The sharp vertex located over the mid-Cen marks the X-axis position of the minimally protected fragment and its Y-axis position (red line) indicates its length.
First, the two double vertical lines of dots seen on either side of the mid-Cen mark the location of two 10-bp cleavage pairs observed using conventional cleavage density histograms.
(A ) The 1-kb interval around the mid-Cen of Cen3.
10.7554/eLife.01861.009 Figure 3. Fragments spanning the mid-Cen are depleted due to high A + T content.
10.7554/eLife.01861.010 Figure 4. Pairs of 10-bp cleavage sites are symmetrically offset around the mid-Cen.
However, the cleavage data alone do not exclude a model in which two oppositely oriented hemisomes flank the mid-Cen.
Strong depletion is seen directly over the middle of aligned CDEIIs, gradually approaching 1 with distance from the mid-Cen.
DOI: http://dx.doi.org/10.7554/eLife.01861.011 10.7554/eLiFigure61.012 Figure 4 figure supplement 2. Cen4 spacing around the mid-Cen position is anomalously short.
Left-right V-plots with MNase and Cse4 ChIP/Input profiles superimposed for all centromeres aligned over the mid-Cen.
The simplest explanation for the cleavage patterns around the mid-Cen is that oppositely oriented hemisomes occupy different members of the cell population.
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CEO of Professional Science Editing for Scientists @ prosciediting.com