c Mouse with cecum ligated at 100%% presented an ischemia of the ligated part.
The ligated probes hybridized with the target genes, were amplified.
For the ligated groups, measurements of ligated ceca were the following: 2 mm (1 2 mm) for the 5 % ligated group, 6 mm (5 7 mm) for the 20%% ligated group, and 21 mm (19 25 mm) for the 100%% ligated group.
The ligated products were then eluted from streptavidin magnetic beads.
The amplified linearized vector and DNA fragments were ligated using Gibson Assembly Master Mix (New England Biolabs), and the ligated DNA products were transformed into E. coli ER2566.
The perfusion ratio per time point was calculated by dividing the perfusion of the ligated (ischemic) by the non-ligated (non-ischemic) hind limb.
The ligated DP was hybridized to its corresponding capture probe (CP) on a DNA array.
The ligated DNAs were purified and subjected to serve as a template for inverse PCR.
We did not observe any difference in amongst the ligated groups (Fig. 5a).
a Blood lactate increased proportionally with the length of the ligated cecum.
