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The phrase "marks the boundary" is correct and usable in written English.
You can use it to refer to something that sets a limit or creates a defined limit between two things. For example, "The river marks the boundary between our two countries."
Exact(60)
This steep bathymetric gradient marks the boundary between continental and oceanic crust.
Lees Ferry also marks the boundary between the river's upper and lower basins, as established by the 1922 compact.
A small wedge-shaped entry, pierced by a big skylight, marks the boundary between inside and out.
Looping westward, it marks the boundary of Tocantins and Maranhão states as far as its junction with the Araguaia River.
The pencil-sized threshold opening marks the boundary between the initial fracture system and the evolving conduit system.
Cheviot Hills, highland range that for more than 30 miles (50 km) marks the boundary between England and Scotland.
Few Brooklynites, for instance, would dispute that DeGraw Street marks the boundary between Cobble Hill and Carroll Gardens.
Very roughly speaking, the difference between direct and indirect statistical evidence marks the boundary between frequentist and Bayesian thinking.
Walking through the streets surrounding the Deportivo, we arrive at an alleyway that marks the boundary of a marginal area known as the Barrio Negro.
What caused the extinction (known as the P-T event because it marks the boundary between the Permian and Triassic periods) is unclear.
Not long after leaving the siding we began a steep climb to the top of a chain of low mountains that marks the boundary between Quebec and Labrador.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com