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To investigate whether Notch signaling is necessary for lineage segregation at the compartment border, clones were induced that express kni, NotchRNAi and GFP. Figure 6I shows a DT clone in a preparation that was stained with antibodies against Kni and Sal (which marks nuclei of DT cells).
Trh (blue) marks nuclei of tracheal cells.
Trh (blue, A ) marks nuclei of tracheal cells.
Trh (blue, D and G ) marks nuclei of tracheal cells.
Similar(55)
To mark the nuclei of transfected cells after extraction, cells were also co-transfected with a plasmid encoding a known chromatin-bound protein GFP-MCM7.
The lymphatic vessels were immunostained in cryosections with anti-Prox1 antibodies, which mark the nuclei of LECs.
Here we conducted a detailed, extensive and comparative analysis of the pattern of active and repressive histone marks in interphase nuclei of phylogenetically important mammalian species: two marsupial models (tammar wallaby and opossum) and an afrotherian (elephant).
Selected sections were labelled with NeuN antibody, which marks selectively the nuclei of mature neurons, using the PAP immunohistochemical detection.
DNA (blue) is stained to mark nuclei.
DAPI (Invitrogen, Carlsbad, CA) staining was used to mark nuclei.
Counterstaining with TOPRO-3 was used to mark nuclei.
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