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Previous lineage analyses based on classical techniques (such as marking a cell and following its progeny) were unable to reconstruct lineage trees at the single cell level due to inherent limitations.
'Hub' genes of a module are often highly biologically relevant, either by playing a functional role (39, 40) or by marking a cell type or cellular process with high specificity (41).
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This plasmid encodes a β-actin promoter driving RFP, followed by the chick U6 promoter driving a short hairpin cassette, such that RFP marks a cell expressing the shRNA.
We marked a cell 'X' when a commonality (a correspondence) was found between a patient recommendation and an intervention component.
Conversely, the white arrowhead in panel b marks a cell that appeared to be CK8-positive and CK5-negative.
This scrambling process often marks a cell for destruction but also plays a key role in many activities throughout the body including cell cell fusion, blood clotting, autoimmune diseases and inflammation.
The first arrows mark a proper spindle formation and division between 68 min and 94 min. The later arrows mark a cell trying to form a spindle but failing starting at 160 min and being stuck in metaphase until the end of the movie.
However, the presence of Ascl1 in a subpopulation of SVZ B cells suggests that Ascl1 may be marking a stem cell population, or at least a population with long term neurogenic potential in this region of the adult brain.
Results of this analysis are consistent with ALDH expression marking a stem cell population.
In humans and many other animal species, brachyury is essential for embryos to develop, marking a layer of cells that will become the skeleton and muscles.
Therefore, the proposed methods may wrongly mark a nearby cell as the host in some rare cases.
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