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Here, we developed a new database containing more SSR markers with unique flanking sequences.
Segregating markers with unique and significant genome scaffold hits (> 90% homology) could be subsequently linked to the physical genome map (Additional file 15).
As expected, some DArT markers with unique DNA sequences gave similar or identical scores in the mapping data, the diversity data, or both.
The 279 markers with unique anchorage were located on 23 AK blocks corresponding to 64 distinct genomic regions (Additional file 7).
Scientific investigations have identified diverse tumor-targeting molecules that can be exploited by nanoparticles to actively target cancer cell-specific markers with unique phenotypes in tumors.
Yet in some regions (chromosome 3D in the PB-KB and PB-KS populations, and chromosome 7D in three populations, etc)., a high number of markers with unique positions can be observed (Additional files 1, 2, 3 and 4).
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This approach averages the results of multiple CIM runs each with different imputation of missing markers and with unique covariate markers, resulting only one or very few markers with detectible signals after.
Therefore, it is necessary to clarify the molecular basis of natural variation and identify molecular markers associate with unique FAs in unrelated soybean germplasm with broad genetic diversity.
Several QTLs related to FAs have been reported [ 3– 9] and a number of molecular markers associated with unique FAs were developed subsequently [ 10– 10].
We genotyped each hybrid for all remaining markers, and then collapsed these loci into a set of marker clusters with unique genotype profiles.
Hence, we collected only the markers with a unique position in the B73 reference genome.
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