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These data build a solid basis for future studies to map and associate markers with single loci or quantitative trait loci for agronomical important traits.
Disappointingly, only 15 markers with single motif of (GATA n were tetranucleotide repeats and nearly no one were used in the wild genetic studies.
Markers with single perfect repeats included 59 dinucleotides, 56 trinucleotides, 64 tetranucleotides, 10 pentanucleotides, 7 hexanucleotides, 3 heptanucleotides, and 1 each of mono, octa and nonanucleotides.
The presented data will allow in the future to map and associate markers with single loci or QTLs for particular traits and molecular cloning of genes controlling these traits.
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Galeano et al. (2009) mapped 288 markers including 118 markers with single-strand conformation polymorphisms.
Mr. Bush typically scrawls over a few selected cards in a black Sharpie marker with single words -- "economy," "jobs" and "small business," for example -- to remind himself to get into his familiar riffs on those subjects, then throws out the rest.
Moreover, 55 InDel markers each with single PCR band identified in this study were experimentally confirmed.
HRM genotyping of the InDel markers each with single PCR band will facilitate quick map construction and gene fine-mapping.
Eight of the 65 markers hybridized with single clones and could not be assembled into contigs or merged with other singletons at stringency 1e-10 (Additional file 3).
Four of the 118 markers hybridized with single clones and the four clones could not be assembled into any contig at the low stringency of 1e-10.
We have not analyzed the prognostic significance of multiple markers as compared with single markers in lymph fluid MM RT-PCR, because of limited number of cases with positive multiple markers.
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