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Finally, a total of 2,961 SSR, RFLP, and IDP markers with precise physical coordinates as well as extra 6,618 bin markers and 1,151,856 SNPs were collected to create a high-quality and density-integrated map (Fig. 5, Additional file 15).
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An important advantage of the MIP technology is the allele discrimination is performed enzymatically and is highly specific, allowing highly multiplexed assays (> 50,000 markers) with very precise quantitation of signals.
However, for regions with a high density of markers, precise marker order has to be taken with care as the marker positions were calculated according to the positions of common markers from different maps and not based on recombination fractions.
Many markers are first made in miniature, with precise scaled-down patterns to determine the optimum layout for minimal yardage; the optimal miniature marker is then used as the guide for making the full-scale cutting marker.
We only considered markers on chromosomes 1 through 22 with precise location mapped.
In some dCAPS markers, generation of digestion-resistant PCR products interferes with precise genotyping.
Sensitivities and specificities with precise 95% confidence intervals (CIs) were calculated for all single and composite markers [ 27].
The skin will be cleaned with alcohol to allow the proper attachment of the markers on precise anatomic sites.
Who can be bothered with precise facts?
RFID wireless markers provided precise three-dimensional positional information and are a potential viable alternative to conventional markers.
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