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CBMCs and PBMCs were stained for activation markers with each of the following monoclonal antibodies (BD Biosciences): CD3-PerCP, CD4-allophycocyanin (APC), HLA-DR-FITC, and QuantiBRITE CD38-PE.
CBMCs and PBMCs were also stained for Treg phenotypic markers with each of the following monoclonal antibodies (BD Biosciences): CD3-PE-Cy7, CD4-allophycocyanin-Cy7 (APC-Cy7), CD25-APC, CD127-PE (Beckman Coulter Biosciences), and CD45RA-PE-Cy5.
Adjustment for BMIz-score almost eliminated the correlations of leptin and HMW adiponectin with the immune mediators, whereas reciprocal associations between the inflammatory markers with each other or IR measures remained partly unaffected (supplementary Table S4).
In total, 2,878 paternal RAD alleles were recovered and these clustered into 285 distinct segregation patterns, hereafter called RAD markers, with each RAD marker containing a minimum of 3 RAD alleles, and this set of RAD markers was used for linkage map construction.
The correlation of the 3 markers with each other was evaluated (Tables 3, 4 and 5).
Correct identification is further ensured by including patient controls corresponding to these variants to serve as markers with each analytical run.
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To fulfill this condition, starting with a single marker, another marker is concatenated to the first marker with each round of the incremental search until the given maximum length L is reached.
Three sections were quantified/embryo for each marker, with each section being 240 µm apart (480 µm total rostral caudal distance); the first section was 300 µm from the most rostral portion of the olfactory bulb.
This report describes the use of a self-encoded marker with each feature on the pattern being augmented with a 2D barcode, tracked by a single analogue in-bore camera attached to the head MR-coil.
Often these lesions also include histologically normal mucosa at the base, offering the opportunity to assess expression of a protein marker with each stage of disease development along the adenoma carcinoma sequence, within a single lesion.
Despite its multiple-copy nature and its concerted mode of evolution [ 36], the ITS2 behaved in the present study just like a "regular" single-copy nuclear marker, with each individual harboring either one or two sequence types.
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