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Interval mapping (IM) and composite IM (CIM) were performed on body weight and body fat traits on a combination of SNP and microsatellite markers, which generated a high-density genotyping panel.
A total of 632 SSR markers, which generated 954 loci mapped onto the consensus map of F.x ananassa, were employed for phylogenetic analysis of 20 wild Fragaria species and 4 F.x ananassa varieties.
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In addition, plants were also screened with the dominant SW13 SCAR marker, which generates a 690 bp PCR amplicon [ 36], using the same PCR thermocycling conditions as those for the CAPS marker, except that the extension time was at 72°C for 1 min. The recombination frequency between markers (SNP ss715641188, SW13) and the I gene in the RIL population was calculated by χ analysis.
The ER– cell population contained bipotent progenitor cells, which generated cell colonies displaying mixed-lineage markers.
The genotype data for each single nucleotide polymorphism (SNP) marker were analyzed using maize SNP3K (Illumina Inc., San Diego, CA), which generated homozygous and heterozygous genotype clusters.
Primers chosen for each marker yielded PCR products compatible with the internal size standard which generated peaks from 50 to 625 bp.
However, 40 redundant markers, which were generated from the same combination and displayed the same genotype, were excluded.
Samples of genomic DNA from 1,306 F2 plants were analyzed in terms of simple sequence length polymorphism and cleaved-amplified polymorphism markers, which were generated on the basis of polymorphism data provided by Cereon (http://www.Arabidopsis.org/Cereon).org/Cereon
In hybrid rye, genomic selection is superior to marker-assisted selection which generates lower accuracies of prediction which are potentially overestimated.
DArT is an array-based genotyping technology which generates DNA markers that are binary and dominant.
We estimated relatedness among the individuals in the mapping population in GEMMA using all markers and the –gk 1 option, which generates a centered relatedness matrix.
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