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Markers were validated using test cultivars containing specific Glu-B1 alleles confirmed by SDS-PAGE and RP-HPLC analysis.
The obtained candidate markers were validated in independent samples covering a wide age range using alternative technologies together with previously proposed DNA methylation, sjTREC, and telomere length markers.
While 116 markers were validated, 70 markers showed one to three alleles, with an average of 0.16 polymorphism information content (PIC) value.
A total of 12 DNA markers were validated with a set of 50 reference strains and isolates, representative of the Streptococcus genus, of closely related species and of microorganisms with matching habitats.
The markers were validated with respect to the tonic behavior (time scale of seconds) of the sleep EEG by establishing that they disambiguated the stages of sleep that are defined solely on the basis of EEG activity.
Moreover, the allele-specific PCR-gel based markers were validated through its application in our marker-assisted selection (MAS) breeding program.
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Generally, the markers are validated in fine mapping studies.
Few preload dependency markers are validated in Children and none for children in spontaneous ventilation (2).
Nineteen LTR sequences were isolated using this new method in tree peony and the applicability of the LTR sequences based markers was validated by further SSAP analysis.
Finally, a set of 11 multiplex panels containing the 30 most informative markers was validated and proposed for B. ruziziensis genetic analysis.
Several of these screening markers are validated, based on minimum performance requirements, at the EU level following ring trials and are included in the Compendium of reference methods for GMO analysis [ 35].
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