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Amplicons that showed polymorphic positions with respect to the BRS sequence, or with critical phylogenetic markers, were replicated at Uppsala University (Sweden) and at the Innovation and Research Centre (San Michele all'Adige, Trento, Italy).
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Each marker was replicated three times as 52 bp long probes randomly printed on the array and all probes were of a length of 52 bp.
These findings were replicated using another marker for thymic-derived Treg, neuropilin 1 [ 70].
The results were replicated at least three times for each marker (from two independent extractions).
Results were replicated.
All experiments were replicated.
All reactions were replicated.
This region was discovered using unions of one, two, or three markers but was replicated only in the single marker analysis (Table 1).
Markers that are replicated in the latter part of the replication cycle are located toward a pole prior to replication, with loci replicated by separate forks being at opposite poles and therefore outside of the average replisome position.
However, very few markers have been replicated consistently between studies.
The same five markers were also replicated in the Porto lab using a first multiplex SNaPshot analysis (Additional file 3).
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