Exact(1)
Primers for these markers were redesigned to amplify significantly shorter PCR products and were combined into 1 multicolor multiplex PCR.
Similar(57)
If samples remained negative, the primers for the STS marker were redesigned so that the new primer sites flanked the existing STS.
In addition, several generic bird microsatellite markers [43] were redesigned (shortened) for aDNA purposes and tested.
Most primer pairs for the markers in the panel were redesigned to yield amplicons of 80 600 bp in multiplex polymerase chain reaction (PCR) and fluorescence-based sequencer analysis, and they were labelled with one of four different fluorescent dyes.
For adaptation to multiplex PCR, most of the primer pairs for the SSR markers developed by USDA were redesigned with the use of the Primer 3 program on the basis of the following parameters: a melting temperature of 50° 60°C (optimum, 55°C); a product size of 80 600 bp; and all other items set to default.
Primers for the Mal d 1.06A SSR marker [ 15] were redesigned according to new sequence data obtained.
Brassieres were redesigned to emphasize the breasts.
Some were redesigned to fit new tastes.
The 3 failed amplicons were redesigned.
Such interventions were redesigned based on process evaluation results.
Most (255 out of 263) of the primer pairs for the USDA SSR markers were therefore redesigned to enhance their suitability for multiplex PCR analysis and to yield PCR products within the detection range of the DNA analyser.
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