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dsDNA size markers were prepared by radiolabeling MspI-digested pBR322 with 32P--dCTP and Klenow DNA polymerase.
As a control, blank nanocapsules without PTX and the fluorescent markers were prepared in a similar manner.
These 50 markers were prepared for the TM and IM strains as listed in Table S3 and Table S4, respectively.
RNA size markers were prepared from Decade Markers (Ambion) following the supplier's instructions and approximately one picomole of radiolabelled size markers was loaded on a gel.
Plasmid-based open circle markers were prepared by cloning PstI-digested lambda DNA (Fermentas International Inc., Canada) into plasmid vector pBluescript II SK+ (Stratagene, USA).
Similar(55)
Blood samples to analyze satiety markers are prepared by adding inhibitors (20 uL AEBSF and 20 uL DPP-4) to one 2 mL tube.
The standard solution containing 24 markers was prepared and diluted with 70% aqueous methanol to appropriate concentrations for the construction of calibration curves.
IHCs for each marker were prepared from at least 10 mammary tumors obtained from 10 different animals.
Haploid MATα yeast libraries containing pathway YACs (pathway libraries, LEU2 marker) and haploid MATa yeast libraries containing cDNA YACs (cDNA libraries, HIS3 marker) were prepared.
Primer aliquots for each marker were prepared by mixing equimolar amounts of appropriate forward and reverse primers in 1× TE (1 mM EDTA, 10 mM Tris-HCl, pH 8.0), and are hereafter referred to as locus-specific primers.
Briefly, donor vesicles comprised of 89 mol% 1-palmitoyl-2-oleoyl phosphatidylcholine (POPC), 1 mol% [H]GalCer, 10 mol% negatively charged dipalmitoyl phosphatidic acid and traces of [C]tripalmitate (nonexchangeable marker) were prepared by sonication in 10 mM sodium phosphate (pH 7.4), 1 mM DTT, 1 mM EDTA, and 0.02% NaN3.
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indicator were prepared
markers were selected
markers were related
markers were fastened
markers were increased
markers were observed
markers were investigated
markers were sorted
markers were determined
markers were considered
markers were designed
markers were put
markers were identified
markers were tested
markers were shared
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