Thirty six pairs of markers were polymorphic (△Tm > 0.3 °C) using HRM technology, such that ~65%% of these markers can be genotyped using this technology (Fig. 2).
Forty of the 52 efficient SSR markers were polymorphic and the polymorphism rate reached 76.92% (40/52) (Table 1).
Among these, 202 markers were polymorphic, amounting to 86.6 % polymorphism.
All of the EPIC markers were polymorphic and showed levels of polymorphism similar to that of the widely used internal transcribed spacer (ITS).
In eight P. umbellatus accessions, eight markers were polymorphic.
Twenty-nine markers were polymorphic and therefore informative for genetic studies while 6 were monomorphic.
Among them, 158 SSR markers were polymorphic between the two parents and were used to construct the genetic linkage map of the RIL population.
All three co-dominant molecular markers linked to the target genes (Xa4, xa5 and Xa21) were used for MAB and the markers were polymorphic between the donor parent IRBB57 and recurrent parent Mangeumbyeo.
For the Yr15 gene carrier chromosome 1B, 29 of the 55 markers were polymorphic.
Second, we genotyped 394 autosomal SNPs (Panel MB1) [34] and determined that 75% of the markers were polymorphic.
Fifteen of these markers were polymorphic.
