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Markers were picked at contig ends for contigs larger than 17 kb, and one marker for contigs smaller than 17 kb.
This can easily be explained in terms of the SNP selection strategy used: markers were picked according to the CEPH HapMap population data using the r2 based method [ 12], ensuring that the CEPH population has best coverage and thus contains more CEPH-specific SNPs.
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For markers falling into 'zero recombination clusters', only one marker was picked that had the most informative meiosis as the representative marker of each cluster for linkage map construction to reduce the power required for computation of linkage.
Colonies which were closest to a marker line were picked to avoid a bias to bigger colonies.
After the transformation, targeted nematodes with a phenotype rescued by the marker cassette were picked after 2 weeks based on their normal movement, and the lines were further cultured.
Markers with a MAF greater than 0.05 were picked as the real markers, and a specific number of QTL, depending on the scenario, with a MAF greater than 0.01 were uniformly picked among the potential QTL.
For each histone marker, 4 different histone patterns were picked up around TSS, which were 20, 15, 10, and 5 bins up and down TSS.
Pig clones were picked if their available sequences matched marker sequences through blast analysis at Sanger Center's website http://www.sanger.ac.uk.sanger.ac.uk
Morex-contigs nearby them were picked up and used to generate new molecular markers potentially linked to HvSGRA gene.
Transformants were picked from LB plates containing ampicillin at a final concentration of 10 µg/mL as a selectable marker.
Colonies were picked and stocked.
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