Simple sequence repeat markers were identified using the Gramene database (http://www.gramene.org/).org/
Among them, 11 markers were identified in three or more environments.
SNP markers were identified by using HRM analysis (Wittwer et al. 2003).
Among these 10 markers, four markers were identified in three or more environments (Table 3; Additional file 1: Table S7).
For the fine mapping of qGCR9, additional SSR markers were identified from the O. sativa Nipponbare genome (http://www.gramene.org).org
Eight putative quantitative trait loci associated with 7 of the 35 new markers were identified within specific families.
Ten putative quantitative trait loci associated with seven of the new markers were identified within specific families.
After screening with 72 primer pairs, 5 AFLP markers were identified that putatively correlated with the target trait.
In addition, 15,106 candidate SSR markers were identified, with 11,037 pairs of PCR primers designed.
For the 10 different M. tuberculosis strains included in the present analysis, 56 polymorphic markers were identified.
In maize only, biallelic GBS markers were identified as follows.
