Highly polymorphic microsatellite markers were genotyped by semi-automated fluorescent genotyping techniques with the ABI3700 machine and Genotyper® software from Applied Biosystems as previously described [23].
To perform interval mapping for the families with significant contrasts, 21 additional markers were genotyped on chromosomes 2, 7, and 27.
Chromosome 7 microsatellite markers were genotyped in all 12 families, and LOD scores were calculated under recessive and dominant models.One family was linked to STBMS1; in three, linkage was significantly excluded; and the remainder were uninformative.
Relevant markers were genotyped on the members of Family 39 who contained informative recombination events, resulting in a further narrowing of LGMD1A to an interval bounded by D5S479 and D5S594, estimated to be 2 Mb in size.
A total of 443,816 markers were genotyped.
Only a few DNA markers were genotyped and tested.
Following quality control, 958 markers were genotyped in both datasets.
Microsatellite markers were genotyped using previously described methods (Wong et al, submitted).
For the 6 remaining genes studied, a total of 48 markers were genotyped in the Norwegian Cognitive NeuroGenetics —NCNG —sample.
Microsatellite markers were genotyped by separating their PCR products on a denaturing polyacrylamide gel as described by Niehuis et al. [33], [94] or on a 4% agarose gel.
Twelve microsatellite markers were genotyped in P. falciparum isolates from Kilifi, Kenya (n = 47) Pikine, Senegal (n = 30); Bakoumba, Gabon (n = 24) were genotyped as previously described [29].
