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Those polymorphic SSR markers were further used for phylogeny analysis and the results showed a complex genetic architecture among genera but a relatively uniform structure in the same genus.
These markers were further verified by accurate mass tandem mass and retention times of available reference standards.
The markers were further used to assess the presence of the two blast R-genes in 434 rice accessions, consisting of 377 Chinese indica cultivars/breeding materials and 57 Chinese japonica cultivars/breeding materials.
The Pi9-Pro and Pi2-LRR markers were further validated for their specificity to Pi2 or Pi9 in monogenic lines harboring blast resistance genes in the Pi2/9 locus, including IRBLz5-CA (Piz-5 = Pi2), IRBL9-W (Pi9), IRBLzt-T (Piz-t) and IRBLz-Fu (Piz) (Tsunematsu et al. 2000).
After identifying rice chromosome 5 as the location of the most significant genetic factor for floury endosperm characteristics, 14 additional SSR markers were further incorporated into the linkage map skeleton to increase marker density (see Table 4; Locus, Ap = 2, 3).
At eight weeks after infection the expression of the M1 markers iNOS and CXCL11 was significantly increased, and these markers were further upregulated at 26 weeks of infection (Fig. 4A).
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Cluster analysis using microsatellite markers was further carried out on the matrix of similarity coefficient with UPGMA method and the phenogram was constructed.
Expression of lymphatic markers was further analyzed by immunological methods.
Therefore, the influence of celastrol on ER stress markers was further observed.
The accurate grouping of markers was further confirmed by comparison to the reference genome (see Additional file 2: Table S2).
These markers are further detailed: This established inflammatory marker has been shown to be increased in individuals with coronary artery disease [ 7, 8].
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