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Cancer-related serum tumor markers were followed for several patients.
Post-PCR steps for all markers were followed as in Zarrei et al. (2014).
The behavior of these keratinocytes expressing fluorescent or FRET markers were followed for multiple days with subcellular resolution using a standard confocal microscope.
To explore the kinetic profile of autophagy and cell death mechanisms the well-known autophagy (such as LC3II and p62) and apoptosis (e.g., procaspase-3, cleaved PARP) markers were followed in time by immunoblotting.
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Detection of the markers was followed by analysis of the cell cycle phases.
The segregation of F3int, F35-1int, F35-2int and 335 SSR markers was followed in the 'Cabernet Sauvignon' × Vitis 'breeding line 20/3' full-sib family [G. Di Gaspero, unpublished data].
For intracellular staining (TCR ζ chain and Foxp3), surface staining for T-cell markers was followed by two washes with the PBS/BSA/NaN3 buffer and by subsequent fixation of the PBMC in 2.5% PFA for 10 min at room temperature in the dark.
To clarify kinetics of the inter-conversion between NSCCs and CSCs, purified NSCCs or CSCs were cultured in a microfluidic chip and the expressions of CD133 cell-surface antigen marker were followed for a period of time.
The REMARK criteria of tumor marker evaluation were followed.
REMARK guidelines for prognostic tumour marker studies were followed (McShane et al, 2005).
Whenever applicable in the study, the REMARK recommendations for reporting of tumor marker studies were followed [ 26].
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