Because the unexpected data points were infrequent, for some time we simply did not notice them, dismissed them as noise, or assumed that they were failed marker assays.
After automatic and manual clustering, 3242 markers (62%) fell into the usable "SNP" marker category, with the remainder being fails, monomorphic or low call-confidence markers.
Many other single gene molecular markers were assessed, but failed to prove clinically relevant [ 9].
The remaining microsatellite markers were either monomorphic, failed to amplify consistently, or were difficult to score unambiguously.
Marker patterns 4 and 5 typically required manual adjustment of locus positions, whereas pattern 6 markers were frequently set as failed.
Ep-CAMhigh/CD49fneg, although expressing some of the stem/progenitor cell markers, were differentiated cells as they failed to form colonies and mammospheres in vitro (data summarized in Table 3).
Seventeen markers were found to be monomorphic and two assays failed; we used the remaining 50 markers in our study.
After linkage analysis, 4550 (Additional file 1: Table S1) of the 6731 (Additional file 2: Table S2) LP markers were mapped onto the genetic map, while the other 2181 markers failed to be linked to any group.
> -wrap-foot> Notes: Polymorphic SLAF marker indicates that the marker has two to four alleles; non-polymorphic SLAF marker indicates that the marker has only one allele After linkage analysis, 2,577 (Additional file 1, Additional file 2) of the 13,635 markers were used to construct genetic linkage maps, while the other 11,058 markers failed to be mapped onto the genetic map.
