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For pure nickel oxide films, the markers were easily incorporated into the growing oxide during very early stages of oxidation and were difficult to detect at the oxide metal interface.
Flow cytometry performed on blood from a mixed infection illustrated the three fluorescence markers were easily distinguishable.
In contrast, the polymorphisms obtained using IP markers were easily discernable on simple 1.5 2.0% agarose gels and would therefore enable rapid screening of large segregating populations.
Twenty-four markers were easily typed on the RH panel by both qPCR and gel electrophoresis, meaning that both agarose gels and dissociation curves resulted in clear differentiation of hamster and buffalo PCR products from individual hybrid clones.
All PCRs were carried out under the following conditions: 60 s denaturation at 95°C, followed by 25 cycles of 95°C for 60 s, annealing at 55°C for 60 s and 72°C for 60 s, and a final extension at 72°C for 5 min. Ten microsatellite markers were easily amplified.
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Nowadays, as NGS technologies are rapidly developed and spread, huge amount of binary single nucleotide polymorphism (SNP) markers is easily generated.
Methylation markers are easily detected in cervical scrapes, with, for example, methylation-specific PCR (MSP).
No objective markers are easily available to differentiate between the entities at first contact.
These two markers are easily accessible and routinely monitored in clinical practice.
These markers are easily detected in this model (CXCL8 not produced by rodents).
Some of these characteristics (e.g. sucrose utilisation and lack of adhesins/antibiotic markers) are easily explained by genome analysis.
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