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All of the designed genomic SSR markers were designated as AHGS (rachis ypogaea genomic SSR) markers.
Purified SMCs expressing multiple SMC markers were designated APSCs (SMA-puromycin selected cells) [26].
AFLP markers were designated by the primer combination used for the amplification and the approximate length of the generated fragment [14].
Markers segregating in both populations received the prefix C. In addition, DArT markers were designated by the enzyme combination used for complexity reduction (BamHI, MseI and RsaI: BMR or HindIII, MseI, RsaI: HMR), the 384-well plate number and the position of the fragment in that plate (i.e., AHMR_04I09).
The newly developed markers were designated FATS markers.
AFLP markers were designated as described (Peters et al., 2001).
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The transfected cells that express red fluorescent protein (RFP) and neomycin-resistant marker were designated MB231RN and were used to derive other sublines used in this study.
Plasmids containing the C. glabrata CEN/ARS and the URA3 auxotrophic marker are designated as the pCU series.
Three types of reproducible marker polymorphism were observed on the PAGE profiles of the CG PCR products: fragment size polymorphism of the targeted gene fragments, additional markers that were designated as "CG accessory markers" generated elsewhere in the profile than the main CG fragments, and single strand conformation polymorphism (SSCP).
In silico data mining of the 26 606 sequences yielded 3800 SSR markers, which were designated as RSS (R aphanus s ativus EST-derived SSR) markers (Supplementary Table S1).
PCR primers were designed from the unique sequences flanking 2,877 SSR loci identified in the TSA contigs for the development of genic-SSR markers and were designated ASSR1 to ASSR2877 (A= 'Arhar', Additional file 3).
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