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InDel markers were created based on polymorphisms between these two ecotypes to meet the demands of researchers.
The REMAP markers were created by combining LTR primers with ISSR primers, as shown in Table 3.
For the VINO-only tree, synthetic markers were created at a small distance from each real marker (0.001 cM).
All PMA1 or pma1-105 sthatns that expressed other markers were created by backcrossing to these original haploids.
Initially, we set the program to call only peaks above 200 reflectance units; thus, bins for markers were created that had at least one peak >200 reflectance units.
For comparison of the filters used to select the most important markers were created two database: one without and one with quality control (QC).
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This pre-defined panel of markers was created for each cross and for each primer pair using the Panel Design program in GeneMarker.
The subset of markers was created by selecting markers from the complete marker set with less than 1% missing data.
LD between markers was created by two bottlenecks to mimic the LD observed at short distances in most species.
To capture the interaction effect between respondent status and baseline functional status on predicting functional status at the final follow-up interview, another set of binary markers was created.
Using the methods described by Karagiannis and Balasubramanian diploid S. pombe strains bearing one wild-type and one synthetically constructed mutant copy of the rpb1 gene (marked with the ura4+ selectable marker) were created (Table S1).
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