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All 16 tumors identified as CIMPW-high using a proposed consensus panel of 5 markers were contained within the CIMP-H group, as well as all 15 tumors containing a BRAF mutation.
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The merging algorithm proceeds from the first to the last bin in the linkage map M that is to be integrated with graph G. Let S v be the set of vertices in G that contain one or more markers in bin v of M. For each vertex w in S v, if all of the markers in w are contained in v, then w remains intact.
We simply searched a neural development marker, NEUROG1, which is contained in the 22nd cluster.
If the cDNA expression cassette is cloned into the NheI site of a mutant loxP vector, both of selectable marker and expression cassette are contained in the region flanked by mutant loxP sites.
All information on the anchor markers, genomic clones, and gene loci is contained in the Q-TARO database (http://qtaro.abr.affrc.go.jp/).jp/
For these latter linkage maps polymorphic markers were limiting, containing less than 200 SSR markers.
Within the interspecific set, the TAMU/UC-Davis Inter RNA-seq markers were selected to contain the maximum number of markers with polymorphism across multiple species (relative to G. hirsutum) as possible and approximately 2000 markers unique to each of the wild species.
The number of intervals that contained markers were counted and the average number of markers per interval was calculated.
The contigs containing markers were used to design primers, employing the Primer3 program (http://bioinfo.ut.ee/primer3/).ee/primer3/
A total of 14,433 contigs containing markers were used to analyse the distribution of the markers on the A2 and D5 genomes.
Three additional microsatellite markers were analyzed in samples that contained enough DNA and showed a partial AI (Additional file 2).
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