Sentence examples for markers were constructed from inspiring English sources
Lentiviral transfer plasmids expressing the red (DsRed2) and green fluorescent protein (EGFP) markers were constructed for in vitro assessment on the basal and mifepristone-induced cell activation levels by FACS analyses.
The strains containing the GFP-tagged polarity markers were constructed by the C-terminal integration of GFP (HIS5-marked PCR fragment).
Knockdown plasmids with puromycin resistant markers were constructed as below.
In this study, 10 small LGs with a majority derived from SNP markers were constructed for the E. oleifera parent.
Two linkage groups, containing 44 markers, were constructed: 20 markers in linkage group (LG) I, and 24 in LG II.
Neighbor-joining trees of sequences for the UPA, COI and LSU markers were constructed based on the maximum-likelihood composite model in MEGA v.4 [ 62].
Using the Genotyping-By-Sequencing approach, a genetic linkage map with an average distance of 0.865 cM between adjacent markers was constructed based on a RIL population in rice.
P-values for phenotypic coefficients were calculated with a two-sided t-test using cor.test function in R. A linkage map consisting of 179 SSR markers (Fig. 4) covering the 12 chromosomes with a total length of 1849.4 cM (an average length of 9.4 cM) between two markers was constructed as described by Jiang et al. (2004).
A framework genetic map comprising 101 loci, which included 71 simple sequence repeat, 21 random amplified polymorphic DNA, eight inter-simple sequence repeat, and one expressed sequence tag markers was constructed previously in this laboratory using the same subset of 250 F7 RILs of this mapping population (Gomez et al. 2010).
A comprehensive genetic map containing 848 markers was constructed for Family 331.
A cassette with puromycin resistance and thymidine kinase selective markers was constructed with flanking Frt5 sites (5'-GAAGTTCCTATTCTTCAAAAGGTATAGGAACTTC-3') [65], [65], and a loxP site, and was subcloned into the BsrDI site (AC026683. position 94582) which is ∼7 kb upstream of the first copy of Snord115.
