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In evaluating whether these markers were consistently depolarization-responsive, we chose to focus on quantifying the fraction of donors that exhibited statistically significant changes upon depolarization, rather than the magnitude of the change (e.g., fold-change values).
Immunofluorescence and realtime PCR analysis showed down-regulation of vascular and stemness markers, while early-to-late cardiac markers were consistently and significantly upregulated in G-FOAM and C-RGD compared to standard CSps culture, suggesting selective commitment towards cardiomyocytes.
His group pinned down the approximate location of the gene by looking to see which chromosome 9 "markers" were consistently found in Tangier patients, but not in unaffected family members--an indication that the gene and marker lie close to one another.
All immunological markers were consistently higher in the HAM classification, while in other classifications these findings were inconsistent.
With this strategy, only 11 new markers were consistently positioned on both parental linkage maps (Additional file 3).
Interestingly, those unassigned markers were consistently observed throughout the patient and healthy control samples with different intensities.
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For example, no pair of markers was consistently outside of the 95% quantile envelope.
Furthermore, the same markers are consistently responsible for the deviations in all five study ponds (Table S1 [see Additional file 1]).
The variation in the relative efficiency of sequencing among DG markers was consistently observed in different RILs and was associated with differences in the GC-content of DG templates.
The axonal injury as assessed with amyloid protein precursor (APP) as a marker was consistently present in all injured rats.
Phenotypic analysis revealed that the CD8 marker was consistently and predominantly observed on gamma/delta + CD3+ cells in the tumour infiltrate, whereas CD4 expression, while generally low, was noted on a significant percentage (median 10%) of LNL gamma/delta + lymphocytes.
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CEO of Professional Science Editing for Scientists @ prosciediting.com