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Blood samples for the determination of sepsis markers were carried out within the first hour of ICU hospitalization.
PCR of microsatellite markers were carried out in Pop2 as previously described [ 15].
These BES microsatellite markers were carried forward for use in construction of the genetic linkage map.
Searches for human nucleotide sequences that were homologous to INT markers were carried out using BLASTN program and public databases as described in Materials and Methods.
Initial screens of the SNP and indel markers were carried out using templates from 'Nasik Red', 'CUDH2150' and the F1 parent of the F2 population.
Polymorphism detection between the two mapping parents and genotyping of the whole mapping population using polymorphic markers were carried out according to the improved SSCP technology described by Li et al. [ 31].
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Expression profiling of the components of Notch, Wnt, SHH and Retinoid signaling along with screening of the neuronal markers was carried out in the NT2 cells and their mutant derivatives.
Amplification and sequencing of the selected bacterial DNA markers was carried out as stated above.
A 10cM primary GWLS of 515 polymorphic markers was carried out in 58 pedigrees (74 nuclear families).
Further staining for surface markers was carried out using APC-conjugated antibodies to CD27, CD45R0, CD62L and CD38, and FITC-conjugated antibodies to CD28, CD45RA, CD95 and the class II HLA-D molecules DR, DP and DQ.
Determination of chromosomal positions of SNP markers was carried out both genetically and physically.
More suggestions(15)
microsatellites were carried
markers were selected
markers were related
markers were fastened
markers were increased
markers were observed
markers were investigated
markers were sorted
markers were determined
markers were considered
markers were designed
markers were put
markers were identified
markers were tested
markers were shared
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