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Several simulation studies in which markers were calibrated on a training set of phenotypes e.g. [ 5- 8] have demonstrated these advantages.
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For each marker, the microscope and the camera were calibrated according to a standardized procedure and these settings were used for all biopsies.
Fractions were calibrated with internal sedimentation markers (alkaline phosphatase (6.1S) and ß-galactosidase (16S)).
Gels were calibrated with pre-stained molecular mass markers (Bio-Rad).
During MS, the ion peaks were calibrated using a mixture of standard peptides as mass markers.
Then the images were calibrated to distance.
Chemical shifts were calibrated against tetramethylsilane (TMS).
The pH electrodes were calibrated regularly.
Western blots were calibrated with protein standards.
Both examiners were calibrated.
Tonometers were calibrated monthly.
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