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However, markers were ascertained for common polymorphisms.
Although these markers were ascertained to recover the largest features of the NNW-SSE axis of genetic differentiation in Europe and not dissect the genetic heterogeneity of Spanish populations, these results are in agreement with results from previous studies using alternative marker sets [9].
Ungenotyped markers were ascertained by two approaches in order to compare methods: 1) imputation and 2) proxy SNPs.
Plasma NGAL, cytokines, blood urea nitrogen (BUN), serum creatinine (Crea) and other laboratory markers were ascertained and ANOVA and Spearman correlation testing performed.
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All DNA markers used were ascertained to be sensitive for indicating bio-geographic ancestry on the level of the four continental regions (Africa, Europe, Asia, and America) expected to have contributed to the current U.S. population.
Genotypes were ascertained from grandparents (P1) and F2 (F1 parental DNA was not available) using microsatellite markers, a genetic sex marker, and SNP markers as described below.
The expected sizes of the amplicons were ascertained by electrophoresis in 1.5% agarose gel with an appropriate molecular size marker (Promega, Brazil).
Socio-demographic and behavioural risk factors were ascertained through personal interview, and infection with hepatitis B and C viruses was determined by their serologic markers.
SSR markers were genotyped on an ABI 3700 XL Capillary DNA Sequencer as previously described [ 47, 49] and SSR allele lengths were ascertained using GeneMapper Applied Biosystemss, Foster City, CA).
Deaths were ascertained from 1970 through 1992.
Reasons for stopping medications were ascertained.
More suggestions(14)
markers were named
markers were confirmed
markers were analysed
markers were checked
markers were assayed
markers were validated
markers were constructed
markers were evaluated
markers were maintained
markers were established
markers were ranked
markers were determined
markers were assembled
markers were identified
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