For some sequences as many as four actors were motion-captured at once.The images of the facial markers were analysed to determine Mr Hanks's expression, and his performance was then "retargeted" onto the faces of the digital models of the film's characters: the conductor, the boy, Father Christmas and so on.
To explore the consequences of Hex-AVE ablation, the expression of primitive streak markers were analysed in Hexdact/+ embryos.
The activation of cardiac-specific markers were analysed by: immunohistochemistry with bright field and immunofluorescent microscopy, electron microscopy, PCR and RT-PCR.
These markers were analysed as categorical variables.
Their differentiation markers were analysed by RT PCR.
The remaining 18 markers were analysed through individual simplex kits.
Cell surface markers were analysed by flow cytometry as described (Kamata et al, 2010).
PCR-amplified microsatellite markers were analysed with an ABI PRISM 310 genetic analyzer and Genescan.
In P4, typical MSC surface markers were analysed by fluorescence cytometry.
LDH release and the expression of osteogenesis-specific markers were analysed after 7 and 14 days.
In addition, conventional inflammatory markers were analysed using multiplexed ELISA (Bio-Plex™ assay).
