Eight of the 12 original significant markers passed the threshold (posterior probabilities of 0.55 to 0.86), and five new significant markers were added (Table 5).
A total of 27 newly-designed microsatellite markers were added to a previously generated map and then localized to specific linkage groups, using a de novo single-pair cross, in order to demonstrate the utility of the tool.
A genetic map for the DH population used in this study was initially published by (Huang et al. 1994), and SSR markers were added to increase the resolution of this framework map (Temnykh et al. 2000).
Subsequently, RFLP and SSR markers were added to the Saltol region, and a test of 54 RILs in a hydroponic screen at the seedling stage revealed that this QTL explained 43% of the variation for seedling shoot Na K ratio in this population (Bonilla et al. 2002).
Then cells were stained in PBS 1× for 10 min at RT, and membrane markers were added for 20 min at 4°C.
A forward step-wise strategy was employed in which the strongest linked markers were added to the background locus model if they improved the fit at the P<0.05 level.
The DArT markers were added to the first genetic linkage map of M. graminicola [14], consisting of 271 AFLP markers, 57 RAPD markers and two markers for the biological traits avirulence (Avr) and mating type (mat).
This result was further confirmed when more fiber EST-SSR markers were added, of which more fiber EST-SSR markers were mapped in At subgenome (207) than in Dt (175) [55].
After blocking incubation, surface markers were added for 15 minutes at 4°C.
RGH markers were added to the genetic map using the program JoinMap 3.0 [ 33].
More than 700 EST- and BAC-based SNP markers were added to the bovine linkage map.
