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GBM cells stained for stemness/differentiation markers were acquired with a BD Facs Aria III (Becton Dickinson).
Electrophoresis reagents, protein markers were acquired from Bio-Rad, Hercules, CA, USA; hyper film and ECL plus reagents were purchased from Amersham Biosciences, Buckinghamshire, UK.
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Fluorescence micrographs for a minimum of 30 fields per GSC marker were acquired using a Leica (DMI3000B) × 20 objective, and images were processed and assembled in Photoshop CS (Adobe).
[F]fluorodeoxyglucose uptake of metastases from germ cell tumours as well as CT scans and serum tumour marker were acquired after 2 3 cycles of induction chemotherapy but before the start of high-dose chemotherapy and CT scans/serum tumour marker were compared with the baseline examinations in 23 patients with relapsed germ cell tumours.
To explain its presence in that area the authors suggested two possibilities: 1) the marker was acquired by Ethiopians through interchanges with Asians or 2) it was present in the ancient Ethiopian population and was carried to Asia by groups who migrated out of Africa.
Furthermore, whenever required, an additional centriolar marker was acquired in the far-red channel by wide-field microscopy to reveal the orientation of centrosomes (e.g. Fig. 1A, upper panel; for details, see supplementary material Fig. S1B and Materials and Methods).
Precise markers of stimulus presentation were acquired using a photodiode.
Pairwise identity-by-state (IBS) distances were calculated between all individuals using these independent SNP markers, and MDS components were acquired using the mds-plot option based on the IBS matrix.
Images of cells positive to HuNu (as a marker of injected ADSCs), and to biglycan and aggrecan (as markers of in vivo differentiation) were acquired by microscopy (Leica TCS SP2 AOBS, Leica Microsystems, Milan, Italy) after specific immunocytochemical staining at 3 months after transplantation.
Additionally, standard behavioral and imaging markers of aMCI disease progression were acquired.
For kinematic analysis, hemocytes were labeled with nuclear and microtubule markers and time-lapse movies were acquired at 10 s/frame.
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