To maximize the chance of a potential microsatellite marker being polymorphic we restricted our search for di-, tetra-,etra-, pentandand hexa- nucleotide motifs that were present repeated at least six times in the contig sequences.
For these tests, we restricted the analyses to markers with minor allele frequencies equal or higher than 5%.
At first, we restrict the number of markers to be less than a certain threshold ((n_text{c})) but still evenly distributed; this allows to quickly estimate the projection maps (P_{omega }).
Thus, although a number of inflammatory markers show restricted expression upon challenge with poly I C, we cannot attribute these differences solely to a lack of responsiveness to acutely induced IFN-I: these animals express lower levels of these markers at baseline, and although poly I C-induced elevatI C-induced possiblelevationselevation is facilitated by the expremainn of IFNAR1.
However, we re-sequenced whole fragments, rather than genotyping a priori identified SNPs, thus included additional markers not restricted by our selection criteria of intermediate variability (see Methods).
The increased expression of the CD40 and CD86 activation markers was restricted to the minute portion of adjuvant-bearing DCs.
Our data suggest that none of these putative stem cell markers are restricted to kidney-specific epithelial stem/progenitor cells, but on the contrary, stem cell markers are always also expressed on differentiated elements.
Effects on these and other markers were restricted to sub-regions and/or sub-sets of neurons in AAV-Tau mice, while most were unaffected in AAV-EGFP or AAV-APP.SLA injected mice (results not shown).
Importantly, most markers normally restricted to specific SCN subdomains showed similar compartmentalization in mutants.
However, the anchorage of AFLP markers is restricted to closely-related species.
