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To identify the relative variant frequencies in the pools of segregants at marker sites, we implemented a custom script to count at each marker site the number of read alignments that support the variant originating from the superior parent (VR1-5B) and the total number of alignments.

Based on the phenotype and marker genotype data, we implemented the multiple-interval mapping method for a preliminary genome-wide scan for QTL affecting the multiple categorical phenotype of cell aggregation.

In addition, we implemented the marker clumping procedure used by PLINK.

For our study, we implemented a multi-marker antibody panel for CTC enrichment which, as previously demonstrated, results in detection of more CTCs than when a single marker is targeted [ 20].

We implemented GBLUP and RKHS using the Bayesian framework by assigning appropriate prior probability distributions to marker effects (see Appendix A).

We implemented worker welfare standards.

So we implemented 'Looking Dumb Range'".

We implemented a two-stage prediction process.

We implemented the algorithm.

So, how would we implement an automaton that evaluates the cell for the presence of two markers, CD45 (marker of all hematopoietic cell) and CD20 (B-cell marker)?

We followed the procedure of Duchesne & Bernatchez [ 47], developed for AFLP markers and implemented in AFLPOP v.1.1.

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