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The expression of CD107a, IFNγ, IL-2, MIP-1β, TNFα and T cell surface markers was quantified separately and in combination.
To test whether exogenous reelin also affects BLBP expression, the number of processes expressing both radial glial markers, vimentin and BLBP, or only one these markers, was quantified (see Table 2).
IHC staining for all hypoxia markers was quantified using H-score.
The expression level of macrophage markers was quantified by taking four images at 10× magnification per injected hemisphere (field).
Staining of pericyte markers was quantified by taking 20 images of the cortex and medulla using 200x total magnification.
The number of RABV ΔG (VSV GRtmC) transduced cells expressing cell-type specific markers was quantified by manually counting the immunohistochemically stained cells in confocal images stacks.
Similar(51)
Immunohistochemical stains of the studied markers were quantified by Image analysis system.
To assess oxidative stress response at the gene expression level, mRNA of 24 markers were quantified in fibroblasts and keratinocytes isolated from the reconstructed skin, at three time points following a DUVR exposure.
Expressions of these three markers were quantified by a pathologist (B.C).
However, the main mesenchymal markers were quantified on the similar level in both cell populations.
Immunoreactive nerve fibres and cell markers were quantified by computerised image analysis (Olympus Analysis Five DP Soft, UK).
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