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To investigate pathology in G2019S-LRRK2 mice (line 340), immunohistochemistry with a number of pathological markers was conducted in the ventral midbrain, striatum and cerebral cortex at 23 24 months of age.
HRM analysis of these markers was conducted between Morex and Barke.
PCR amplification of SNP and InDel markers was conducted according to the methods described by Lin et al. [ 30].
A detailed analysis of 73 clones identified by 12 SSR markers was conducted to confirm the accuracy of the data obtained from the BAC pool screening.
One of the earliest efforts towards the construction of comparative plant maps using molecular markers was conducted in the Solanaceae family.
Genotyping of the plant material from this study with background and Mt-FTQTL6-associated markers was conducted as described in Tayeh et al. [ 39].
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RF regressions of metabolites on markers were conducted using the "Random Forest" package of the R-software [42].
Over the last years, a few genetic characterization studies using molecular markers were conducted with J. curcas [ 5– 8].
BLASTX search for the resulting non-redundant markers were conducted to the rice, Brachypodium and sorghum genes with E value < 10−5.
Predictions of the secondary structure of β-barrel proteins, essential to estimate the localization of the cladistic markers were conducted with the PSIPRED server (http://bioinf.cs.ucl.ac.uk/psipred/ [last accessed 2011 Dec 18], Bryson et al. 2005).
Restriction digestions for both markers were conducted in a 10-μl volume with 1× buffer (New England Biolabs), 1 μL PCR product, 5 10 units of enzyme (New England Biolabs), and 100 μg/ml Bovine Serum Albumin (when required).
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