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And the presence of apoptotic markers was analyzed with annexin V-FITC/PI stains.
Cell proliferation was measured using bromo-deoxyuridine and immunohistochemistry. Amount of CD47 and stem cell markers was analyzed by western blot analysis and location by immunofluorescence microscopy.
The applicability of the IT markers was analyzed in other potato genotypes, and their transferability was studied in other Solanum species: section Archaesolanum (5 species), sect.
The polymorphism information of STS and CAPS markers was analyzed by PowerMarker v.3.25 (Liu and Muse 2005) to investigate the number of alleles and polymorphism information content (PIC) per marker.
Thereafter, expression of DCs surface markers was analyzed by FACS or functional assays were performed.
Expression of mesenchymal and hepatic markers was analyzed by RT-PCR, Western blot and immunohistochemistry.
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Distortions of the array of nanodots used as spot markers were analyzed near the triple junction.
Finally, the genes included in an interval of +/− 50 kb around the markers were analyzed.
A series of markers were analyzed during the differentiation of the macrophage cells to osteoclasts.
Secondarily, the distension properties of different frames by tracking markers were analyzed.
Genes underlying these QTLs (+/−50 kb from the significant markers) were analyzed.
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