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That this collapsing of an entire people into some exaggerated markers was achieved so effortlessly is what hurts the most.
Detection of the described markers was achieved with FAM/MGB probes (Applied Biosystems): Nkx2.5 (Myh6657783_m1), Myh6 (Mm00440354_m1), Brachyury (Mm00436877_m1).
Successful amplification of all five markers was achieved in only 51% of samples.
Amplification for all five markers was achieved in 23 out of 25 (92%) of the normal tissue samples.
Amplification of the five markers was achieved in all 10 MSS tumour samples and matching normal DNA.
Successful amplification of the five markers was achieved for both the tumour and normal DNA in all 15 cases.
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They did not understand that physical improvements were often experienced before the recommended laboratory health markers were achieved.
These results indicate that a more uniform depth of sequencing among DG markers is achieved using randomly sheared DG templates that are within the same size distribution.
Aligning with the emerging phenotype, a conceivable reduction in the expression of a number of inflammatory markers is achieved, in partial analogy to the situation with anti-inflammatory drugs treatment [ 63].
The prediction of migration time of electroosmotic flow (EOF) marker was achieved by applying artificial neural networks (ANN) model based on principal component analysis (PCA) and standard normal distribution simulation to the input variables.
Transformants were selected and maintained on SD plates (SD broth with 2% (w/v) purified agar) with appropriate auxotrophic supplements, and recycling of the URA3 marker was achieved by plating cells on SD plates containing 0.1% (w/v) 5-fluoroorotic acid (5-FOA) and uridine (25 μg/ml).
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