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Cells were transfected with pIRESneo- dZIP13-myc and constructs of ER/Golgi markers using lipofectamine (Invitrogen).
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MAO-B cells were transfected with 3X-FLAG vector containing an insertion of human parkin cDNA (gift of Keio University School of Medicine Tokyo, Japan) and a neomycin selection marker (Clontech) using Lipofectamine 2000 reagent (Invitrogen).
This DNA was co-transfected with DNA containing a hygromycin resistance marker in MCF-7 Tet-On® cells using Lipofectamine 2000 (Invitrogen, Carlsbad, CA).
siRNA and mRNA reporters were transfected using Lipofectamine 2000.
Cells transfected using Lipofectamine 2000, a commercially available transfection reagent, were included as controls.
The 293T cells were transfected with pcDNA3-KDM5B-Myc-His WT and mutants using Lipofectamine 2000.
Transfection was performed using Lipofectamine 2000 reagent (Invitrogen) according to the manufacturer's instructions.
The recombinant plasmids were exogenously expressed in HEK 293T cells by transient transfection using lipofectamine 3000.
Oligonucleotide transfection was performed using Lipofectamine 2000 reagents (Invitrogen, USA) according to the manufacturer's instructions.
Transfections were carried out using Lipofectamine (Invitrogen).
Cells were transfected using Lipofectamine 2000 (Invitrogen).
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