Sentence examples for markers using e-PCR from inspiring English sources

We attempted to place these markers using e-PCR, permitting 1 gap and 1 mismatch [ 24].

The positions of SSR markers designed from the sequences of genotypes other than Nipponbare were estimated using e-PCR to search Nipponbare genomic sequences (IRGSP Pseudomolecules build 4.0).

Finally, all STS markers obtained were searched against the sequences exhibiting tandem repeat sequence lengths variation within WGS sequences using e-PCR [ 23].

And then the co-cultured tubular epithelial cells were harvested for detecting mRNA expression of EMT- and fibrosis-related markers using qRT-PCR (n = 3).

Furthermore, we identified and analyzed these genome-wide indel polymorphisms among the populations employed using an e-PCR strategy, with the aim of enhancing the efficiency of maize genetic research and molecular marker assisted breeding.

Sequence information for RFLP and SSR markers used in QTL mapping allowed us to determine their physical positions through the use of BLASTN and e-PCR searches.

Specifically, when developing new markers for mapping studies, e-PCR can be used to test potential primers in various ways before actually incurring the expense of oligonucleotide synthesis.

PCR primers used for nuclear markers used in this study.

Additional file 3: PCR conditions of the microsatellite markers used for paternity analyses.

Additional file 1: List and PCR conditions of the microsatellite markers used for population structure analysis.

Most PCR based DNA markers used for this study have already been published: Markers with an asterisk in Fig. 2 were designed by RGP (http://rgp.dna.affrc.go.jp/E/publicdata/caps/index.html).html