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Different DNA markers to genotype Trypanosoma cruzi are now available.
The approach we're taking in my lab is to use a RAD tagging or GBS type of approach in a mapping family to get mapped markers and then get baits made for those map markers and proceed with those as being the most useful markers to genotype in the natural populations.
We then used the polymorphic markers to genotype the five mapping populations.
CO, was responsible of DNA extraction and the selection of markers to genotype and helped to perform linkage analysis.
We used 12 highly polymorphic microsatellite markers to genotype each sampled individual (Aguirre et al. 2014; Table S1).
For Lotus, the first genetic maps were produced using amplified fragment length polymorphism and microsatellite markers to genotype F2 offspring.
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The genotype of each recombination bin was represented by a bin marker, which was defined as a set of genetically linked SNPs of the same genotype that were collected into a single bin group with a physical position located in the middle of each recombination bin, which was used as a virtual bin marker to genotype the population.
Additionally, we used the marker to genotype populations of E. necator from different Vitis spp. from throughout the USA.
Nuclear markers used to genotype all Oculina spp. samples in this study.
Additional file 3: Table of microsatellite and SNP markers used to genotype congenic strains.
What are the allele-specific molecular markers used to genotype the plant shown in Figure 1B?
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