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We then used special software that can "join the dots" represented by the markers, to create a stick figure of the athlete.
The new genomic sequencing discovery makes it possible to develop genetic markers to create cabernet sauvignon grapevines with traits individually suited for specific growing conditions in specific vineyard locations throughout the world.
They would then have students color in the images with markers to create Cinco de Mayo murals.
The 10-year-old used colored markers to create her well-conceived artwork, which features a U.S. flag outlining all 50 of the United States, with a Native American girl and a Pilgrim boy flanking a turkey inscribed with words that describe the holiday.
The final linkage map is shown in Fig. 2 and 3. Twenty chromosomes had sufficient anchoring markers to create integrated LGs following the procedures outlined in Supplement S4.
Additionally, the methylation status of two other genes, APC and MLH1, were determined and we added them to the CIMP markers to create a methylation index of seven genes.
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The GLO I PCR product was digested with HindIII and cloned in the vector pGEM-7zfαNeoα (containing neomycin phosphotransferase as the selection marker) to create pGEM-7zfαNeoα-LdGLOI containing the neomycin phosphotransferase gene.
The plasmid CIp20- PYK1 was then transformed into CLM29-2, selecting for the URA3 marker, to create the strain CLM45-9.
In this newly created cell line the fhuA gene was replaced with dhfr (Trimethoprim resistance marker) to create the W3110B55 cell line.
This procedure was then repeated by starting selection at the second marker to create a new sample map and continuing to initiate selections at subsequent markers until the sth marker.
In order to eliminate position effects in our analysis and focus on the pleiotropic unintended effects, we employed the Cre/l ox system to excise the ABF3 transgene from the site of insertion, leaving behind the selectable marker, to create control plant lines.
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